NDLI: Effects of protease-activated receptors (PARs) on intracellular calcium dynamics of acinar cells in rat lacrimal glands

Content Provider	Springer Nature Link
Author	Oikawa, Makoto Sai, Tomoyuki Kimura, Katsura Kamada, Yuki Tamagawa, Yasuri Kurosaka, Daijiro Satoh, Yoh ichi
Copyright Year	2013
Abstract	Protease-activated receptors (PARs) represent a novel class of seven transmembrane domain G-protein coupled receptors, which are activated by proteolytic cleavage. PARs are present in a variety of cells and have been prominently implicated in the regulation of a number of vital functions. Here, lacrimal gland acinar cell responses to PAR activation were examined, with special reference to intracellular Ca2+ concentration ([Ca2+]i) dynamics. In the present study, detection of acinar cell mRNA specific to known PAR subtypes was determined by reverse transcriptase polymerase chain reaction. Only PAR2 mRNA was detected in acinar cells of lacrimal glands. Both trypsin and a PAR2-activating peptide (PAR2-AP), SLIGRL-NH2, induced an increase in [Ca2+]i in acinar cells. The removal of extracellular Ca2+ and the use of Ca2+ channel blockers did not inhibit PAR2-AP-induced [Ca2+]i increases. Furthermore, U73122 and xestospongin C failed to inhibit PAR2-induced increases in [Ca2+]i. The origin of the calcium influx observed after activated PAR2-induced Ca2+ release from intracellular Ca2+ stores was also evaluated. The NO donor, GEA 3162, mimicked the effects of PAR2 in activating non-capacitative calcium entry (NCCE). However, both calyculin A (100 nM) and a low concentration of Gd3+ (5 μM) did not completely block the PAR2-AP-induced increase in [Ca2+]i. These findings indicated that PAR2 activation resulted primarily in Ca2+ mobilization from intracellular Ca2+ stores and that PAR2-mediated [Ca2+]i changes were mainly independent of IP3. RT-PCR indicated that TRPC 1, 3 and 6, which play a role in CCE and NCCE, are expressed in acinar cells. We suggest that PAR2-AP differentially regulates both NCCE and CCE, predominantly NCCE. Finally, our results suggested that PAR2 may function as a key receptor in calcium-related cell homeostasis under pathophysiological conditions such as tissue injury or inflammation.
Starting Page	463
Ending Page	476
Page Count	14
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	140
Issue Number	4
e-ISSN	1432119X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-03-06
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lacrimal gland Protease-activated receptors Intracellular calcium Confocal microscopy RT-PCR Biomedicine general Cell Biology Biochemistry Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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