NDLI: Zooming in on single active genes in living mammalian cells

Content Provider	Springer Nature Link
Author	Yunger, Sharon Kalo, Alon Kafri, Pinhas Sheinberger, Jonathan Lavi, Erez Shav Tal, Yaron
Copyright Year	2013
Abstract	The kinetic aspects of RNA polymerase II as it transcribes mRNA have been revealed over the past decade by use of live-cell imaging and kinetic analyses. It is now possible to visualize polymerase molecules in action, and most importantly to detect and follow the mRNA product as it is generated in real time on active genes. Questions such as the speed at which mRNAs are transcribed or the number of polymerases running along a particular gene can be addressed at high temporal resolution. These kinetic studies highlight the tight regulation that genes encounter when moving between active and inactive states, and ultimately will shed light on the kinetic aspects of transcription of genes under perturbed states. The scientific pathway along which these findings were unearthed begins with the imaging of the action of hundreds of genes working in concert in fixed cells. The state of the art has reached the capability of analyzing the transcription of single alleles in living mammalian cells.
Starting Page	71
Ending Page	79
Page Count	9
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	140
Issue Number	1
e-ISSN	1432119X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-06-09
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Transcription RNA polymerase II Live-cell imaging Nucleus Promoter Single allele Biomedicine general Cell Biology Biochemistry Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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