NDLI: Simultaneous blocking of IL-6 and IL-8 is sufficient to fully inhibit CAF-induced human melanoma cell invasiveness

Content Provider	Springer Nature Link
Author	Jobe, Njainday Pulo Rösel, Daniel Dvořánková, Barbora Kodet, Ondřej Lacina, Lukáš Mateu, Rosana Smetana, Karel Brábek, Jan
Copyright Year	2016
Abstract	Tumour microenvironment plays a critical role in cell invasion and metastasis. To investigate the role of cancer-associated fibroblasts (CAFs) in melanoma cell invasiveness, we used 3D spheroid invasion assay. The effect of conditioned media from normal fibroblasts and CAFs cultivated alone or co-cultivated with melanoma cells on BLM or A2058 melanoma spheroid invasion was analysed. We found that conditioned media from CAFs and CAFs co-cultured with melanoma cells, especially, promote invasion and migration, without significant effect on melanoma cell proliferation. We further analysed the expression of pro-invasive cytokines IL-8 and IL-6 in media and found that melanoma cells are dominant producers of IL-8 and fibroblasts are dominant producers of IL-6 in 2D monocultures, while co-cultivation of CAFs with melanoma cells induces production/secretion of IL-6 and IL-8 into the media. The analyses of IL-6 levels in 3D cultures and human melanoma samples, however, revealed that at least in some cases IL-6 is also produced directly by melanoma cells. Analysis of the role of IL-6 and IL-8 in CAF-induced melanoma invasion, using neutralising antibodies, revealed that simultaneous blocking of IL-6 and IL-8 is sufficient to fully inhibit CAF-induced human melanoma cell invasiveness. In summary, these experiments indicate the important role of CAFs and IL-8 and IL-6 cytokines in melanoma cell invasiveness.
Starting Page	205
Ending Page	217
Page Count	13
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	146
Issue Number	2
e-ISSN	1432119X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-04-21
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cancer microenvironment Cancer-associated fibroblasts 3D culture of melanoma cells Cytokine Chemokine Invasiveness Biomedicine general Cell Biology Biochemistry Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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