NDLI: Mitotic lymphoma cells are characterized by high expression of phosphorylated ribosomal S6 protein

Content Provider	Springer Nature Link
Author	Egervári, Gábor Márk, Ágnes Hajdu, Melinda Barna, Gábor Sápi, Zoltán Krenács, Tibor Kopper, László Sebestyén, Anna
Copyright Year	2011
Abstract	Growth factors and mitogens influence signaling pathways and often induce the activity of p70S6 kinase (p70S6K), which in turn phosphorylates the ribosomal S6 protein (S6). Although recent data are rather conflicting, the overall view suggests that phosphorylated S6 is a regulator of global protein synthesis, cell proliferation, cell size and glucose homeostasis. In the present work, emphasis was given to cell cycle-dependent activation of S6 focusing mainly on human lymphoid and lymphoma cells. Paraffin-embedded human tissue blocks from lymph node and different tumor biopsies as well as in vitro cell lines were investigated by immunohistochemistry, immunocytochemistry, flow cytometry and Western blotting using antibodies directed against phospho-S6, phospho-mTOR, phospho-p70S6K and phospho-Histone H3. To enrich the cell number in different phases of the cell cycle, nocodazole, staurosporine or rapamycin were used in cell cultures. We observed strong phospho-S6 positivity by immunostainings in the dividing lymphoid cells of reactive lymph nodes and in lymphoma cells cultured in vitro. Phospho-S6 protein levels were shown to be elevated throughout mitosis in lymphoma cells; however, the high expression of phospho-S6 in mitotic cells was not a general hallmark of tumor cell types studied so far: phospho-S6-negative mitotic cells were detected in several carcinoma and sarcoma biopsies. These observations may have practical implications as they raise the possibility to consider p70S6K and/or S6 as a potential therapeutic target—besides mTOR—in certain lymphomas and perhaps in clinical immunosuppression.
Starting Page	409
Ending Page	417
Page Count	9
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	135
Issue Number	4
e-ISSN	1432119X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-20
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	S6 activity Mitosis mTOR activity Cell cycle progression Lymphomas Anatomy Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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