NDLI: Effect of ageing on the murine venous circulation

Content Provider	Springer Nature Link
Author	Hemmeryckx, Bianca Emmerechts, Jan Bovill, Edwin G. Hoylaerts, Marc F. Lijnen, H. R.
Copyright Year	2012
Abstract	The effect of ageing on the morphology of veins, venous valves and arteries was investigated in male wild-type mice using an adapted procedure with injection of a silicone polymer Microfil® that preserves morphology of the vasculature. Throughout the hind limb the arterial, but not the venous, lumen area and wall thickness were significantly greater in 24-month as compared to 10-week-old C57BL/6 mice. Venous valves were most frequently located at the sapheno-femoral vein junction in the lower extremities, and appeared thicker at the base supported by structurally intact collagen fibers, and thinner towards the proximal end of the valve leaflet, with less organized collagen. Overall, valves were less supported by structurally intact collagen at 24 months as compared to 10 weeks. Endothelial expression of CD31, endothelial protein C receptor or von Willebrand factor (VWF) was not affected by age, while thrombomodulin expression was lower in aged versus young arteries. At both ages, expression of VWF was lower at venous valves versus veins. Evaluation of the blood coagulation profile revealed that aged mice had shortened prothrombin time, elevated plasma levels of factor (F)VII, FVIII and VWF and increased neutrophil and platelet counts. Thus, our data indicate that in mice with ageing, venous valves become more fragile, in association with a procoagulant and inflammatory blood phenotype. Taken together, we found that the procoagulant state in ageing, is accompanied by mild vascular changes.
Starting Page	537
Ending Page	546
Page Count	10
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	137
Issue Number	4
e-ISSN	1432119X
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-18
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ageing C57BL/6J mice Vasculature Venous valves Anatomy Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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Effect of ageing on the murine venous circulation