NDLI: Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates

Content Provider	Springer Nature Link
Author	Ma, Lu Wu, Sheng Mei Huang, Jing Ding, Yi Pang, Dai Wen Li, Lijia
Copyright Year	2007
Abstract	Semiconductor nanocrystals, also called quantum dots (QDs), are novel inorganic fluorophores which are brighter and more photostable than organic fluorophores. In the present study, highly dispersive QD-labeled oligonucleotide (TAG)8 (QD-deoxyribonucleic acid [DNA]) conjugates were constructed via the metal-thiol bond, which can be used as fluorescence in situ hybridization (FISH) probes. FISH analysis of maize metaphase chromosomes using the QD-DNA probes showed that the probes could penetrate maize chromosomes and nuclei and solely hybridized to complementary target DNAs. Compared with the conventional organic dyes such as Cy3 and fluorescein isothiocyanate, this class of luminescent labels bound with oligonucleotides is brighter and more stable against photobleaching on the chromosomes after FISH. These results suggest that QD fluorophores may be a more stable and useful fluorescent label for FISH applications in plant chromosome mapping considering their size-tunable luminescence spectra.
Starting Page	181
Ending Page	187
Page Count	7
File Format	PDF
ISSN	00095915
Journal	Chromosoma
Volume Number	117
Issue Number	2
e-ISSN	14320886
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-11-29
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Eukaryotic Microbiology Animal Genetics and Genomics Human Genetics Biochemistry Developmental Biology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Genetics (clinical)

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