NDLI: Condensin I binds chromatin early in prophase and displays a highly dynamic association with Drosophila mitotic chromosomes

Content Provider	Springer Nature Link
Author	Oliveira, Raquel A. Heidmann, Stefan Sunkel, Claudio E.
Copyright Year	2007
Abstract	The condensed state of mitotic chromosomes is crucial for faithful genome segregation. Key factors implicated in the formation of mitotic chromosomes are the condensin I and II complexes. In Drosophila, condensin I appears to play a major role in mitotic chromosome organization. To analyze its dynamic behavior, we expressed Barren, a condensin I non-Structural Maintenance of Chromosomes subunit, as a fully functional enhanced green fluorescent protein (EGFP) fusion protein in the female and followed it during early embryonic divisions. We find that, in Drosophila, Barren-EGFP associates with chromatin early in prophase concomitantly with the initiation of chromosome condensation. Barren-EGFP loading starts at the centromeric region from where it spreads distally reaching maximum accumulation at metaphase/early anaphase. Fluorescence Recovery After Photobleaching analysis indicates that most of the bound protein exchanges rapidly with the cytoplasmic pool during prometaphase/metaphase. Taken together, our results suggest that in Drosophila, condensin I is involved in the initial stages of chromosome condensation. Furthermore, the rapid turnover of Barren-EGFP indicates that the mechanism by which condensin I promotes mitotic chromosome organization is inconsistent with a static scaffold model.
Starting Page	259
Ending Page	274
Page Count	16
File Format	PDF
ISSN	00095915
Journal	Chromosoma
Volume Number	116
Issue Number	3
e-ISSN	14320886
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-02-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Eukaryotic Microbiology Animal Genetics and Genomics Human Genetics Biochemistry Developmental Biology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Genetics (clinical)

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