NDLI: An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae

Content Provider	Springer Nature Link
Author	Borges, Vanessa Smith, Duncan J. Whitehouse, Iestyn Uhlmann, Frank
Copyright Year	2013
Abstract	Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for their alignment on the spindle apparatus and segregation in mitosis. Budding yeast cohesin first associates with chromosomes in G1. Then, during DNA replication in S-phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to make cohesin’s DNA binding resistant to destabilization by the Wapl protein. Whether stabilization of cohesin molecules that happen to link sister chromatids is sufficient to build sister chromatid cohesion, or whether additional reactions are required to establish these links, is not known. In addition to Eco1, several other factors contribute to cohesion establishment, including Ctf4, Ctf18, Tof1, Csm3, Chl1 and Mrc1, but little is known about their roles. Here, we show that each of these factors facilitates cohesin acetylation. Moreover, the absence of Ctf4 and Chl1, but not of the other factors, causes a synthetic growth defect in cells lacking Eco1. Distinct from acetylation defects, sister chromatid cohesion in ctf4Δ and chl1Δ cells is not improved by removing Wapl. Unlike previously thought, we do not find evidence for a role of Ctf4 and Chl1 in Okazaki fragment processing, or of Okazaki fragment processing in sister chromatid cohesion. Thus, Ctf4 and Chl1 delineate an additional acetylation-independent pathway that might hold important clues as to the mechanism of sister chromatid cohesion establishment.
Starting Page	121
Ending Page	134
Page Count	14
File Format	PDF
ISSN	00095915
Journal	Chromosoma
Volume Number	122
Issue Number	1-2
e-ISSN	14320886
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-01-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sister chromatid cohesion Cohesin Eco1 Chl1 Ctf4 S. cerevisiae Cell Biology Developmental Biology Biochemistry Human Genetics Animal Genetics and Genomics Eukaryotic Microbiology
Content Type	Text
Resource Type	Article
Subject	Genetics Genetics (clinical)

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