NDLI: Induction and repair of DNA double-strand breaks assessed by gamma-H2AX foci after irradiation with pulsed or continuous proton beams

Content Provider	Springer Nature Link
Author	Zlobinskaya, O. Dollinger, G. Michalski, D. Hable, V. Greubel, C. Du, G. Multhoff, G. Röper, B. Molls, M. Schmid, T. E.
Copyright Year	2012
Abstract	In particle tumor therapy including beam scanning at accelerators, the dose per voxel is delivered within about 100 ms. In contrast, the new technology of laser plasma acceleration will produce ultimately shorter particle packages that deliver the dose within a nanosecond. Here, possible differences for relative biological effectiveness in creating DNA double-strand breaks in pulsed or continuous irradiation mode are studied. HeLa cells were irradiated with 1 or 5 Gy of 20-MeV protons at the Munich tandem accelerator, either at continuous mode (100 ms), or applying a single pulse of 1-ns duration. Cells were fixed 1 h after 1-Gy irradiation and 24 h after 5-Gy irradiation, respectively. A dose–effect curve based on five doses of X-rays was taken as reference. The total number of phosphorylated histone H2AX (gamma-H2AX) foci per cell was determined using a custom-made software macro for gamma-H2AX foci counting. For 1 h after 1-Gy 20-MeV proton exposures, values for the relative biological effectiveness (RBE) of 0.97 ± 0.19 for pulsed and 1.13 ± 0.21 for continuous irradiations were obtained in the first experiment 1.13 ± 0.09 and 1.16 ± 0.09 in the second experiment. After 5 Gy and 24 h, RBE values of 0.99 ± 0.29 and 0.91 ± 0.23 were calculated, respectively. Based on the gamma-H2AX foci numbers obtained, no significant differences in RBE between pulsed and continuous proton irradiation in HeLa cells were detected. These results are well in line with our data on micronucleus induction in HeLa cells.
Starting Page	23
Ending Page	32
Page Count	10
File Format	PDF
ISSN	0301634X
Journal	Radiation and Environmental Biophysics
Volume Number	51
Issue Number	1
e-ISSN	14322099
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-07
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	RBE Protons DNA DSB repair Gamma-H2AX foci Pulsed irradiation Environmental Monitoring/Analysis Biophysics and Biological Physics Environmental Physics Effects of Radiation/Radiation Protection Ecosystems
Content Type	Text
Resource Type	Article
Subject	Biophysics Environmental Science Radiation

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