NDLI: Bystander effects and compartmental stress response to X-ray irradiation in L929 cells

Content Provider	Springer Nature Link
Author	Temelie, Mihaela Stroe, Daniela Petcu, Ileana Mustaciosu, Cosmin Moisoi, Nicoleta Savu, Diana
Copyright Year	2016
Abstract	Bystander effects are indirect consequences of radiation and many other stress factors. They occur in cells that are not directly exposed to these factors, but receive signals from affected cells either by gap junctions or by molecules released in the medium. Characterizing these effects and deciphering the underlying mechanisms involved in radiation-induced bystander effects are relevant for cancer radiotherapy and radioprotection. At doses of X-ray radiation 0.5 and 1 Gy, we detected bystander effects as increased numbers of micronuclei shortly after the treatment, through medium transfer and by co-cultures. Interestingly, bystander cells did not exhibit long-term adverse changes in viability. Evaluation of several compartmental stress markers (CHOP, BiP, mtHsp60, cytHsp70) by qRT-PCR did not reveal expression changes at transcriptional level. We investigated the involvement of ROS and NO in this process by addition of specific scavengers of these molecules, DMSO or c-PTIO in the transferred medium. This approach proved that ROS but not NO is involved in the induction of lesions in the acceptor cells. These results indicate that L929 cells are susceptible to stress effects of radiation-induced bystander signaling.
Starting Page	371
Ending Page	379
Page Count	9
File Format	PDF
ISSN	0301634X
Journal	Radiation and Environmental Biophysics
Volume Number	55
Issue Number	3
e-ISSN	14322099
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-03-30
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bystander effect DNA damage X-rays Compartmental stress response Biophysics and Biological Physics Effects of Radiation/Radiation Protection Environmental Physics Environmental Monitoring/Analysis Ecosystems
Content Type	Text
Resource Type	Article
Subject	Biophysics Environmental Science Radiation

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