NDLI: Single exposure to radiation produces early anti-angiogenic effects in mouse aorta

Content Provider	Springer Nature Link
Author	Soucy, Kevin G. Attarzadeh, David O. Ramachandran, Raghav Soucy, Patricia A. Romer, Lewis H. Shoukas, Artin A. Berkowitz, Dan E.
Copyright Year	2010
Abstract	Radiation exposure can increase the risk for many non-malignant physiological complications, including cardiovascular disease. We have previously demonstrated that ionizing radiation can induce endothelial dysfunction, which contributes to increased vascular stiffness. In this study, we demonstrate that gamma radiation exposure reduced endothelial cell viability or proliferative capacity using an in vitro aortic angiogenesis assay. Segments of mouse aorta were embedded in a Matrigel-media matrix 1 day after mice received whole-body gamma irradiation between 0 and 20 Gy. Using three-dimensional phase contrast microscopy, we quantified cellular outgrowth from the aorta. Through fluorescent imaging of embedded aortas from Tie2GFP transgenic mice, we determined that the cellular outgrowth is primarily of endothelial cell origin. Significantly less endothelial cell outgrowth was observed in aortas of mice receiving radiation of 5, 10, and 20 Gy radiation, suggesting radiation-induced endothelial injury. Following 0.5 and 1 Gy doses of whole-body irradiation, reduced outgrowth was still detected. Furthermore, outgrowth was not affected by the location of the aortic segments excised along the descending aorta. In conclusion, a single exposure to gamma radiation significantly reduces endothelial cell outgrowth in a dose-dependent manner. Consequently, radiation exposure may inhibit re-endothelialization or angiogenesis after a vascular injury, which would impede vascular recovery.
Starting Page	397
Ending Page	404
Page Count	8
File Format	PDF
ISSN	0301634X
Journal	Radiation and Environmental Biophysics
Volume Number	49
Issue Number	3
e-ISSN	14322099
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-04-18
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ecosystems Environmental Physics Environmental Monitoring/Analysis Biophysics and Biological Physics Effects of Radiation/Radiation Protection
Content Type	Text
Resource Type	Article
Subject	Biophysics Environmental Science Radiation

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Early hematopoiesis inhibition under chronic radiation exposure in humans

Maternal occupational exposure to ionizing radiation and birth defects

A mouse model of cytogenetic analysis to evaluate caesium137 radiation dose exposure and contamination level in lymphocytes

Acknowledgement to Referees

Acknowledgement to referees

Acknowledgement to referees

Modeling hematopoietic system response caused by chronic exposure to ionizing radiation

Radionuclide transfer to reptiles

Enhanced intestinal tumor multiplicity and grade in vivo after HZE exposure: mouse models for space radiation risk estimates

Single exposure to radiation produces early anti-angiogenic effects in mouse aorta

Similar Documents

Early hematopoiesis inhibition under chronic radiation exposure in humans

Maternal occupational exposure to ionizing radiation and birth defects

A mouse model of cytogenetic analysis to evaluate caesium137 radiation dose exposure and contamination level in lymphocytes

Acknowledgement to Referees

Acknowledgement to referees

Acknowledgement to referees

Modeling hematopoietic system response caused by chronic exposure to ionizing radiation

Radionuclide transfer to reptiles

Enhanced intestinal tumor multiplicity and grade in vivo after HZE exposure: mouse models for space radiation risk estimates

Single exposure to radiation produces early anti-angiogenic effects in mouse aorta