NDLI: Adsorption of neutral polymers on negatively charged liposomes. A novel quantitative method to measure the rate of polymer adsorption on the liposomal surface

Content Provider	Springer Nature Link
Author	Dékány, Gy. Csóka, I. Erös, I.
Copyright Year	2001
Abstract	We studied the adsorption of two neutral polymers [poly(vinyl pyrrolidone) and poly(vinyl alcohol) (PVA)] on negatively charged liposomes composed of 25:2:3 (molar ratio) soy lecithin/dicetyl phosphate/cholesterol.The liposomes were prepared in buffered solution at pH 7.4 and were mixed with the solution of the polymers in the desired polymer/lipid ratios. Adsorption was measured by determination of the equilibrium bulk concentration of the polymer. Protamine hydrochloride was used to aggregate the liposomes with polymers adsorbed on their surface and to facilitate their separation from the equilibrium bulk solution. In the case of PVA, quantitative adsorption measurements with a specific reagent were possible. Adsorption isotherms were recorded at 25 ± 0.2 °C. It was concluded that adsorbed and nonadsorbed PVA molecules are in equilibrium even at low polymer/lipide ratios. The results were confirmed by dynamic laser light scattering, X-ray diffraction and thermal activity monitoring experiments.
Starting Page	966
Ending Page	975
Page Count	10
File Format	PDF
ISSN	0303402X
Journal	Colloid and Polymer Science
Volume Number	279
Issue Number	10
e-ISSN	14351536
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-10-01
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Colloid and Surface Chemistry Materials Chemistry Physical and Theoretical Chemistry Polymers and Plastics

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