NDLI: Impact of spinach consumption on DNA stability in peripheral lymphocytes and on biochemical blood parameters: results of a human intervention trial

Content Provider	Springer Nature Link
Author	Moser, Beate Szekeres, Thomas Bieglmayer, Christian Wagner, Karl Heinz Mišík, Miroslav Kundi, Michael Zakerska, Oliwia Nersesyan, Armen Kager, Nina Zahrl, Johann Hoelzl, Christine Ehrlich, Veronika Knasmueller, Siegfried
Copyright Year	2011
Abstract	A controlled intervention trial was conducted to assess the impact of spinach consumption on DNA stability in lymphocytes and on health-related biochemical parameters.The participants (n = 8) consumed homogenised spinach (225 g/day/person) over a period of 16 days. DNA migration was monitored in single cell gel electrophoresis—comet assays under standard conditions, which reflect single- and double-strand breaks, after treatment of nuclei with lesion-specific enzymes (formamidopyrimidine glycosylase, FPG and endonuclease III, ENDO III) and after treatment of intact cells with H2O2 before, during and after intervention.While no reduction in DNA damage was observed under standard conditions after different time intervals of spinach intake, other endpoints, namely ROS sensitivity and DNA migration attributable to the formation of oxidatively damaged DNA bases (i.e. pyrimidines-ENDO III-sensitive sites and purines-FPG sensitive sites) were reduced 6 h after consumption of the first portion and after 11 days of continuous consumption. In the case of ENDO III-sensitive sites, also after 16 days, a decrease in comet formation was observed. At the end of a 40 days washout period, the DNA stability parameters were not significantly different from the background values. Other biochemical parameters which were significantly altered by spinach intake were the folate (+27%) and homocysteine (−16%) concentrations in blood, and it was found in an earlier human study that folate may prevent oxidative damage to DNA bases.Taken together, our results show that moderate consumption of spinach causes protection against oxidative DNA damage in humans and that this phenomenon is paralleled by alterations of health-related biochemical parameters.
Starting Page	587
Ending Page	594
Page Count	8
File Format	PDF
ISSN	14366207
Journal	European Journal of Nutrition
Volume Number	50
Issue Number	7
e-ISSN	14366215
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Intervention trial Spinach DNA damage Antioxidant Nutrition
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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