NDLI: Isoelectric focusing of β-glucosidase humic-bound activity in semi-arid Mediterranean soils under management practices

Content Provider	Springer Nature Link
Author	Doni, Serena Macci, Cristina Chen, Haiyan Masciandaro, Grazia Ceccanti, Brunello
Copyright Year	2011
Abstract	Changes in β-glucosidase enzyme–humic complexes and conventional parameters (pH, total organic C, total N, water-soluble C, and bulk density) were studied in an almond-cropped soil prone to erosion under a rehabilitation practice. The experimental plan included three soil slopes (0%, 2%, and 6%) and two type of fertilization (organic and mineral), with sampling of rhizosphere and inter-row soils. The enzyme humic complexes were extracted by pyrophosphate, purified by ultrafiltration of the organic extracts on molecular mass exclusion membranes (mol wt > 104) and fractionated by isoelectric focusing technique (IEF). The IEF on polyacrylamide rod gels with a restricted pH gradient ranging between 6.0 and 4.0 gave five humic bands on the basis of the little differences of their electric charges (pI). Under both organic and mineral fertilization, β-glucosidase activity bound to the fractionated humic substances, especially in the pH range 4.5–4.2 of the rhizosphere soil, was higher than that of the inter-row soil. This also occurred in 6% slope where the enzyme activity was lower than in soil with lower slopes. The higher number of the β-glucosidase active humic bands in rhizosphere than inter-row soil, particularly for the 0% slope, may be due to the presence of humic molecules capable of preserving the enzyme molecules in the active form, other than to the higher microbial activity synthesizing and releasing the tested enzymes.
Starting Page	183
Ending Page	190
Page Count	8
File Format	PDF
ISSN	01782762
Journal	Biology and Fertility of Soils
Volume Number	48
Issue Number	2
e-ISSN	14320789
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-09-14
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	β-Glucosidase activity β-Glucosidase–humic complexes Organic management Mineral management Isoelectric focusing Soil Science & Conservation Agriculture
Content Type	Text
Resource Type	Article
Subject	Agronomy and Crop Science Soil Science Microbiology

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