NDLI: Effect of hypercapnia on intracellular pH regulation in a rainbow trout hepatoma cell line, RTH 149

Content Provider	Springer Nature Link
Author	Huynh, Khuong Tuyen Baker, Daniel W. Harris, Robert Church, John Brauner, Colin J.
Copyright Year	2011
Abstract	Fish exposed to elevated water CO2 experience a rapid increase in blood CO2 levels (hypercapnia), resulting in acidification of both intra- and extra-cellular compartments. While the mechanisms associated with extracellular pH regulation have been well explored, much less is known about intracellular pH (pHi) regulation. There is great interest in developing non-animal models for research. One such model is the rainbow trout hepatoma cell line (RTH 149), which has been used to study a wide range of topics; however, no studies have investigated its potential use in pHi regulation. Employing the pH-sensitive fluoroprobe BCECF, the present study examined pHi regulation in RTH 149 under normocapnia and during extracellular acidification induced by either elevated CO2 or 1 M HCl. During exposure to hypercapnia, RTH 149 cells were acidified without recovery as long as the elevated CO2 was maintained. In addition, rates of pHi recovery from NH4Cl-induced acidosis were significantly lower in cells exposed to hypercapnia or HCl compared to that in normocapnic cells, indicating that elevated CO2 indirectly impeded pHi recovery through a reduction in pHe and/or pHi. Moreover, pHi regulation in RTH 149 was EIPA-sensitive, suggesting that an NHE may be involved. Overall, RTH 149 may have the potential for identifying transporters likely to play a role in pHi regulation in fish. However, it should not be used as a complete replacement for in vivo studies, especially to quantify acid–base regulatory ability at whole animal level, since RTH 149 appeared to have enhanced pHi recovery rates relative to primary hepatocytes.
Starting Page	883
Ending Page	892
Page Count	10
File Format	PDF
ISSN	01741578
Journal	Journal of Comparative Physiology B
Volume Number	181
Issue Number	7
e-ISSN	1432136X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-05-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rainbow trout hepatoma cell line RTH 149 Hypercapnia Carbon dioxide (CO2) pHi regulation Zoology Human Physiology Animal Physiology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Biochemistry Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Endocrinology

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