NDLI: Transport of sodium and chloride across earthworm skin in vitro

Content Provider	Springer Nature Link
Author	Krumm, S. Goebel Lauth, S. G. Fronius, M. Clauss, W.
Copyright Year	2005
Abstract	We present a new invertebrate model for the study of epithelial sodium transport in tight epithelia, the earthworm integument. Dissected segments of earthworm integument were mounted in modified Ussing chambers and perfused with either pond water (PW) or earthworm ringer solution (ERS) on the apical side. In order to investigate ion transport under near-in vivo physiological conditions, measurements were performed under current-clamp conditions by monitoring the transepithelial potential (V T), as well as the transepithelial resistance (R T). These were recorded continuously and the virtual short circuit current (I SC) was calculated. The integument has a high transepithelial resistance (R T=9,037±502 Ω cm2 for PW, n=24, and 11,055±1,320 Ω cm2 for ERS, n=32). V T was −3.7±2.2 mV for PW (n=24) and −1.5±1.0 mV for ERS (n=32), and I SC was −0.57±0.30 μA/cm2 for PW (n=24) and −0.44±0.24 μA/cm2 for ERS (n=32). Only under PW, but not under ERS conditions, was there a pronounced inhibition of I SC by low doses of amiloride or its analogues phenamil and benzamil. The resistance of the paracellular pathway was found to be very high. The terrestrial oligochaete Lumbricus seems especially adapted to the environmental conditions because it has an ultra-tight integument and a very fast up- and down-regulation of apical Na+ channels.
Starting Page	601
Ending Page	608
Page Count	8
File Format	PDF
ISSN	01741578
Journal	Journal of Comparative Physiology B
Volume Number	175
Issue Number	8
e-ISSN	1432136X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-08-25
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Earthworm Sodium Chloride Absorption Electrical potential difference Animal Physiology Zoology Human Physiology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Biochemistry Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Endocrinology

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