NDLI: Endogenous lactate transport in Xenopus laevis oocyte: dependence on cytoskeleton and regulation by protein kinases

Content Provider	Springer Nature Link
Author	Tosco, Marisa Faelli, Alide Gastaldi, Giulia Paulmichl, Markus Orsenigo, Maria vella
Copyright Year	2007
Abstract	Carbon flux in Xenopus laevis oocyte is glycogenic and an endogenous monocarboxylate transporter is responsible for intracellular lactate uptake. The aim of the present study was to determine if direct activation of protein kinases C and A modulates the activity of lactate transporter, as well as to investigate the possible role of cytoskeleton in these regulatory phenomena. The modulation was studied in isolated Xenopus oocytes of stage V–VI by measuring 14C-lactate uptake, both in the absence and in the presence of cytoskeletal-perturbing toxins. We found that the basal lactate transporter activity depends on the integrity of the cytoskeleton since it is partially inhibited by cytoskeleton disorganisation. Both PKA and PKC activation caused a significant decrease in transport activity and this decrease could be blocked by specific protein kinase inhibitors. The evidenced effects were not additive. Transport inhibition was annulled by agents that destabilize actin filaments or microtubules. We conclude that both protein kinases A and C, whose effects are mediated by cytoskeleton, negatively regulate the endogenous lactate transporter of Xenopus oocyte, suggesting that these kinases may have a role in the control of cytosolic pyruvate/lactate pool in the oocyte.
Starting Page	457
Ending Page	463
Page Count	7
File Format	PDF
ISSN	01741578
Journal	Journal of Comparative Physiology B
Volume Number	178
Issue Number	4
e-ISSN	1432136X
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-01-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Xenopus oocyte Lactate transport PKA PKC Cytoskeleton Animal Physiology Zoology Human Physiology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Biochemistry Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Endocrinology

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Endogenous lactate transport in Xenopus laevis oocyte: dependence on cytoskeleton and regulation by protein kinases.

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