NDLI: PACAP-38 is a chemorepellent and an agonist for the lysozyme receptor in Tetrahymena thermophila

Content Provider	Springer Nature Link
Author	Mace, S. R. Dean, J. G. Murphy, J. R. Rhodes, J. L. Kuruvilla, H. G.
Copyright Year	2000
Abstract	Pituitary adenylate cyclase activating peptide (PACAP-38) is a peptide hormone which functions in many mammalian systems, including the nervous and digestive systems. Using in vivo behavioral studies, we have found that this hormone functions as a chemorepellent in Tetrahymena thermophila with an EC50 of 10 nM. Cells previously adapted to PACAP-38 were found to be adapted to lysozyme, and vice versa. Furthermore, the in vivo behavioral activity of PACAP-38 was blocked by addition of the anti-lysozyme receptor antibody, 5545. Chemorepellent activity of PACAP-38 was also inhibited by the addition of neomycin sulfate (inhibition constant K i=0.080 μmol · l−1), a competitive inhibitor of lysozyme binding to its receptor. PACAP-38 is a more potent and specific agonist for the lysozyme receptor than either intact lysozyme or CB2, a 24-amino acid fragment of lysozyme.
Starting Page	39
Ending Page	43
Page Count	5
File Format	PDF
ISSN	03407594
Journal	Journal of Comparative Physiology A
Volume Number	186
Issue Number	1
e-ISSN	14321351
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-01-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Behavioral Neuroscience Animal Science and Zoology Ecology, Evolution, Behavior and Systematics

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PACAP-38 is a chemorepellent and an agonist for the lysozyme receptor in Tetrahymena thermophila