NDLI: The spatial resolution of dual-tracer fluorescence thermometry in volumetrically illuminated channels

Content Provider	Springer Nature Link
Author	Kim, Myeongsub Yoda, Minami
Copyright Year	2013
Abstract	This study estimates the spatial resolution and accuracy of dual-tracer fluorescence thermometry (DFT) for measuring water temperature fields in channels where the entire channel is illuminated as is typical in microfluidics. Temperature fields are measured in heated laminar Poiseuille flow through a 1 mm2 channel. The working fluid, an aqueous solution of the temperature-sensitive fluorophores fluorescein (Fl) and sulforhodamine B (SrB), is volumetrically illuminated over the entire channel cross-section at a wavelength of 514 nm, and the temperature of the solution is estimated from images of the longer-wavelength fluorescence from Fl and SrB. These temperature data are compared with numerical simulations of the same flow where the heat transferred to the water is estimated from independent measurements of wall surface temperature to determine the accuracy and the spatial resolution of the DFT results. The results suggest that temperature measurements in the volumetrically illuminated channel are significantly corrupted by the fluorescence emissions from beyond the focal plane. A model based on the point spread function for an aberration-free lens is employed to estimate the effect of the background “noise,” i.e., the signal from beyond the object plane, on the accuracy of these DFT measurements. The results show that this background is about 30 times the signal from the focal plane. Further experiments where the channel is illuminated by a light sheet over about 40 % of the channel cross-section give estimates of the water temperature field that are on average within about 0.3 °C of the numerical predictions at an in-plane spatial resolution of 50 μm. The model is used to estimate the signal-to-background ratio for this case, as well as for a variety of commercially available microscope objectives.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF
ISSN	07234864
Journal	Experiments in Fluids
Volume Number	55
Issue Number	1
e-ISSN	14321114
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-12-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Engineering Fluid Dynamics Fluid- and Aerodynamics Engineering Thermodynamics, Heat and Mass Transfer
Content Type	Text
Resource Type	Article
Subject	Fluid Flow and Transfer Processes Physics and Astronomy Mechanics of Materials Computational Mechanics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Dual-tracer fluorescence thermometry measurements in a heated channel

Single- and dual-band collection toluene PLIF thermometry in supersonic flows

The saturation of the fluorescence and its consequences for laser-induced fluorescence thermometry in liquid flows

Spatial resolution correction for hot-wire anemometry in wall turbulence

Interfacial shear stress measurement using high spatial resolution multiphase PIV

The use of vitamins as tracer dyes for laser-induced fluorescence in liquid flow applications

Particle tracer response across shocks measured by PIV

Spatial resolution and dissipation rate estimation in Taylor--Couette flow for tomographic PIV

Spatial filtering improved tomographic PIV

The spatial resolution of dual-tracer fluorescence thermometry in volumetrically illuminated channels

Similar Documents

Dual-tracer fluorescence thermometry measurements in a heated channel

Single- and dual-band collection toluene PLIF thermometry in supersonic flows

The saturation of the fluorescence and its consequences for laser-induced fluorescence thermometry in liquid flows

Spatial resolution correction for hot-wire anemometry in wall turbulence

Interfacial shear stress measurement using high spatial resolution multiphase PIV

The use of vitamins as tracer dyes for laser-induced fluorescence in liquid flow applications

Particle tracer response across shocks measured by PIV

Spatial resolution and dissipation rate estimation in Taylor--Couette flow for tomographic PIV

Spatial filtering improved tomographic PIV

The spatial resolution of dual-tracer fluorescence thermometry in volumetrically illuminated channels