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Near-wall hindered Brownian diffusion of nanoparticles examined by three-dimensional ratiometric total internal reflection fluorescence microscopy (3-D R-TIRFM)

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Near-wall hindered Brownian diffusion of nanoparticles examined by three-dimensional ratiometric total internal reflection fluorescence microscopy (3-D R-TIRFM)

Content Provider	Springer Nature Link
Author	Kihm, K. D. Banerjee, A. Choi, C. K. Takagi, T.
Copyright Year	2004
Abstract	A three-dimensional nanoparticle tracking technique using ratiometric total internal reflection fluorescence microscopy (R-TIRFM) is presented to experimentally examine the classic theory on the near-wall hindered Brownian diffusive motion. An evanescent wave field from the total internal reflection of a 488-nm bandwidth argon-ion laser is used to provide a thin illumination field on the order of a few hundred nanometers from the wall. Fluorescence-coated polystyrene spheres of 200±20 nm diameter (specific gravity=1.05) are used as tracers and a novel ratiometric analysis of their images allows the determination of fully three-dimensional particle locations and velocities. The experimental results show good agreement with the lateral hindrance theory, but show discrepancies from the normal hindrance theory. It is conjectured that the discrepancies can be attributed to the additional hindering effects, including electrostatic and electro-osmotic interactions between the negatively charged tracer particles and the glass surface.
Starting Page	811
Ending Page	824
Page Count	14
File Format	PDF
ISSN	07234864
Journal	Experiments in Fluids
Volume Number	37
Issue Number	6
e-ISSN	14321114
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-09-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Fluid Flow and Transfer Processes Physics and Astronomy Mechanics of Materials Computational Mechanics

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