NDLI: Droplet streams for serial crystallography of proteins

Content Provider	Springer Nature Link
Author	Weierstall, U. Doak, R. B. Spence, J. C. H. Starodub, D. Shapiro, D. Kennedy, P. Warner, J. Hembree, G. G. Fromme, P. Chapman, H. N.
Copyright Year	2007
Abstract	Serial diffraction of proteins requires an injection method to deliver analyte molecules—preferably uncharged, fully hydrated, spatially oriented, and with high flux—into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.
Starting Page	675
Ending Page	689
Page Count	15
File Format	PDF
ISSN	07234864
Journal	Experiments in Fluids
Volume Number	44
Issue Number	5
e-ISSN	14321114
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-11-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Theoretical and Applied Mechanics Thermodynamics Measurement Science, Instrumentation Industrial Chemistry/Chemical Engineering Fluids Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Fluid Flow and Transfer Processes Physics and Astronomy Mechanics of Materials Computational Mechanics

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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