NDLI: Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus

Content Provider	Springer Nature Link
Author	Chen, Yanjie Zhang, Quanqi Qi, Jie Wang, Zhigang Wang, Xubo Sun, Yeying Zhong, Qiwang Li, Shuo Li, Chunmei
Copyright Year	2010
Abstract	The symmetrical body of flatfish larvae changes dramatically into an asymmetrical form after metamorphosis. The molecular mechanisms responsible for this change are poorly understood. As an initial step to clarify these mechanisms, we used representational difference analysis of cDNA for the identification of genes active during metamorphosis in the Japanese flounder, Paralichthys olicaceus. One of the up-regulated genes was identified as creatine kinase muscle type 1 (CK-M1). Sequence analysis of CK-M1 revealed that it spanned 1 708 bp and encoded a protein of 382 amino acids. The overall amino acid sequence of the CK-M1 was highly conserved with those of other organisms. CK-M1 was expressed in adult fish tissues, including skeletal muscle, intestine and gill. Whole mount in-situ hybridization showed that the enhanced expression of CK-M1 expanded from the head to the whole body of larvae as metamorphosis progressed. Quantitative analysis revealed stage-specific high expression of CK-M1 during metamorphosis. The expression level of CK-M1 increased initially and peaked at metamorphosis, decreased afterward, and finally returned to the pre-metamorphosis level. This stage-specific expression pattern suggested strongly that CK-M1 was related to metamorphosis in the Japanese flounder. Its specific role in metamorphosis requires further study.
Starting Page	558
Ending Page	564
Page Count	7
File Format	PDF
ISSN	02544059
Journal	Chinese Journal of Oceanology and Limnology
Volume Number	28
Issue Number	3
e-ISSN	19935005
Language	English
Publisher	SP Science Press
Publisher Date	2010-05-20
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	CK-M1 metamorphosis gene expression Paralichthys olivaceus Oceanography
Content Type	Text
Resource Type	Article
Subject	Water Science and Technology Oceanography

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