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| Content Provider | Springer Nature Link |
|---|---|
| Author | Liu, Huaide Liu, Mei Wang, Baojie Jiang, Keyong Jiang, Shan Sun, Shujuan Wang, Lei |
| Copyright Year | 2010 |
| Abstract | In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons, and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall, the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp. |
| Starting Page | 808 |
| Ending Page | 814 |
| Page Count | 7 |
| File Format | |
| ISSN | 02544059 |
| Journal | Chinese Journal of Oceanology and Limnology |
| Volume Number | 28 |
| Issue Number | 4 |
| e-ISSN | 19935005 |
| Language | English |
| Publisher | SP Science Press |
| Publisher Date | 2010-06-29 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | intestinal microbial diversity PCR-DGGE Kuruma shrimp Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Water Science and Technology Oceanography |
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