NDLI: Survey of cyanomyovirus abundance in Shantou coastal waters by g20

Content Provider	Springer Nature Link
Author	Li, Chuanbiao Ding, Jun Zhou, Lizhen Zhang, Zhao Li, Shengkang Liu, Wenhua Wen, Xiaobo
Copyright Year	2015
Abstract	To understand the genetic diversity and population changes in cyanophages in the coastal waters of Shantou, northeast South China Sea, we used the capsid assembly protein gene g20 as a marker of the abundance and phylogeny of natural cyanomyovirus communities. The abundance of total viruses, heterotrophic bacteria, and picophytoplankton in the coastal waters was monitored with flow cytometry. Hydrological parameters (NO 3 − , NO 2 − , NH3, soluble reactive phosphorus, total dissolved nitrogen, total dissolved phosphorus, dissolved oxygen, chemical oxygen demand, temperature, salinity, and chlorophyll a concentration) and microbial abundance (total viruses, total bacteria, Prochlorococcus, Synechococcus, and eukaryotes) were measured in the upper and lower layers at four sampling sites in the research area. In the direct viral counts, cyanomyoviruses accounted for 1.92% to >10% of the total viral community. A phylogenetic analysis showed that the g20 sequences in the Shantou coastal waters were very diverse, distributed in eight distinct operational taxonomic units, including the newly formed Cluster W. The g20 gene copies inferred from real time PCR assay indicated that cyanomyoviruses were correlated significantly with the heterotrophic bacteria numbers and the nitrate and chlorophyll a concentrations. These results suggest that cyanomyoviruses are ubiquitous and are an abundant component of the virioplankton in Shantou coastal waters.
Starting Page	604
Ending Page	615
Page Count	12
File Format	PDF
ISSN	02544059
Journal	Chinese Journal of Oceanology and Limnology
Volume Number	33
Issue Number	3
e-ISSN	19935005
Language	English
Publisher	Science Press
Publisher Date	2015-03-25
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	cyanomyovirus g20 Shantou coastal water Oceanography
Content Type	Text
Resource Type	Article
Subject	Water Science and Technology Oceanography

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