NDLI: Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

Content Provider	Springer Nature Link
Author	Zhan, Wenbin Li, Yongqin Sheng, Xiuzhen Xing, Jing Tang, Xiaoqian
Copyright Year	2010
Abstract	We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder (Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405), we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral supernatant, skin nodules, gut, gill, kidney, spleen, stomach, heart, and liver of Japanese flounder. We compared the DNA sequence with 14 MCP nucleotide sequences from GenBank, including Megalocytivirus (OFIV and RSIV), Iridovirus (CzIV and WIV), Ranavirus (TFV and FV3), and Lymphocystivirus (8 LCDV). Based on the alignment, we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH, we noted the presence of LCDV in the skin nodules, gut, gill, spleen, stomach, and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s black rockfish (Sebastes schlegeli Higendorf), redwing sea robin (Lepidotrigla microptera Günther) and turbot (Scophthalmus maximus) using the one-step and nested PCR, suggesting the target genes can be widely detected in fish using this method.
Starting Page	1213
Ending Page	1220
Page Count	8
File Format	PDF
ISSN	02544059
Journal	Chinese Journal of Oceanology and Limnology
Volume Number	28
Issue Number	6
e-ISSN	19935005
Language	English
Publisher	SP Science Press
Publisher Date	2010-06-24
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	lymphocystis disease virus PCR in-situ hybridization Paralichthys olivaceus Sebastes schlegeli Lepidotrigla microptera Scophthalmus maximus Oceanography
Content Type	Text
Resource Type	Article
Subject	Water Science and Technology Oceanography

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© rajshahi university zoological society the lymphocystis diseases in the olive flounder, paralichthys olivaceus.

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