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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shao, Zhanru Liu, Fuli Li, Qiuying Yao, Jianting Duan, Delin |
| Copyright Year | 2014 |
| Abstract | Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments, and consequently may have highly efficient ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) activity for carbon assimilation. In our study, we cloned the full-length Rubisco gene from S. japonica (SJ-rbc). It contained an open reading frame for a large subunit gene (SJ — rbcL) of 1 467 bp, a small subunit gene (SJ-rbcS) of 420 bp, and a SJ-rbcL/S intergenic spacer of 269 bp. The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa, 5.81 and 15.84 kDa, 4.71, respectively. After induction with 1 mmol/L isopropyl-β-D-thiogalactopyranoside for 5 h and purification by Ni2+ affinity chromatography, electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein. Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night. This observation implied that Rubisco played a role in normal gametophytic growth and development. In juvenile sporophytes, mRNA levels of SJ-rbcL, carbonic anhydrase, Calvin-Benson-Bassham cycle-related enzyme, and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance. Similarly, expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m2·s). Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements. |
| Starting Page | 377 |
| Ending Page | 389 |
| Page Count | 13 |
| File Format | |
| ISSN | 02544059 |
| Journal | Chinese Journal of Oceanology and Limnology |
| Volume Number | 32 |
| Issue Number | 2 |
| e-ISSN | 19935005 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2013-12-28 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Saccharina japonica Rubisco photosynthetic genes transcription protein expression carboxylase activity Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Water Science and Technology Oceanography |
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