NDLI: Properties of proteolytic toxin ofVibrio anguilolarum from diseased flounder

Content Provider	Springer Nature Link
Author	Zhao lan, Mo Shi yong, Chen Pei jun, Zhang
Copyright Year	2002
Abstract	Extracellular products (ECP) produced byVibrio anguillarum strain M3 originally isolated from diseased flounder (Paralichthys olivaceus) were prepared. ECP of M3 showed gelatinase, casinase, amylase and haemolytic activity on agarose plates. High protease activity against azocasin was detected. Bacterium M2 showed highest growth and protease activity at 25°C. The protease present in ECP showed maximal activity at pH 8 and 55°C; was completely inactivated by application of 80°C heat for 30 min; was completely inhibited by EDTA and HgCl2, and was partially inhibited by PMSF, SDS, MnCl2 and iodoacetic acid; but not inhibited by CaCl2 and MgCl2. The ECP was toxic to flounder fish at LD50 values of 3.1 μg protein/g body weight. The addition of HgCl2 and application of heat at 50°C decreased the lethal toxicity of ECP. When heated at 100°C, ECP lethality to flounder was completely inhibited. After intramuscular injection of ECP into flounder, it showed evident histopathological changes including necrosis of muscle, extensive deposition of haemosiderin in the spleen, dilated blood vessels congested with numerious lymphocytes in the liver. These results showed that ECP protease was a lethal factor produced by the bacteriumV. anguillarum M3.
Starting Page	316
Ending Page	322
Page Count	7
File Format	PDF
ISSN	02544059
Journal	Chinese Journal of Oceanology and Limnology
Volume Number	20
Issue Number	4
e-ISSN	19935005
Language	English
Publisher	Science Press
Publisher Date	2002-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Vibrio anguillarum extracellular products protease toxicity Oceanography
Content Type	Text
Resource Type	Article
Subject	Water Science and Technology Oceanography

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