NDLI: Plasmon-enhanced fluorescence near metallic nanostructures: biochemical applications

Content Provider	Springer Nature Link
Author	Goldys, E.M. Barnett, A. Xie, F. Drozdowicz Tomsia, K. Gryczynski, I. Matveeva, E.G. Gryczynski, Z. Shtoyko, T.
Copyright Year	2007
Abstract	Amplification of fluorescence is a nanoscale phenomenon which is particularly pronounced in close proximity to metal nanostructures. Due to its sharp distance dependence, it is ideally suited to monitor biorecognition reactions. Using this effect we have been able to demonstrate ultrasensitive bioassays. Two types of metal nanostructures have been employed, nanometric silver islands deposited over an ultrathin metal mirror and silver fractal structures. For the first type, metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs and an immunoassay (model assay for rabbit IgG or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results show that SIFs alone (on a glass surface not coated with metal) enhance the immunoassay signal approximately 3 to 10-fold. Using a metal mirror instead of glass as support for SIFs leads to up to 50-fold signal enhancement. The second type of metal nanostructures, silver fractals, were produced by electrochemical reduction of silver nitrate deposited on sapphire covered with a thin conductive film of indium tin oxide. These structures were used as a substrate for a model rabbit IgG bioassay. The fluorescence resulting from the binding of antibody labeled with Rhodamine was highly nonuniform with distinctive hot spots. These highly fluorescent regions were correlated with areas of higher Ag thickness and coverage. Such high values of fluorescence amplification in both types of nanostructures have been interpreted by using time-resolved fluorescence data and by considering the radiative properties of plasmons in the environments which promote plasmon coupling.
Starting Page	265
Ending Page	271
Page Count	7
File Format	PDF
ISSN	09478396
Journal	Applied Physics A
Volume Number	89
Issue Number	2
e-ISSN	14320630
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-06-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Operating Procedures, Materials Treatment Surfaces and Interfaces, Thin Films Characterization and Evaluation of Materials Nanotechnology Optical and Electronic Materials Condensed Matter
Content Type	Text
Resource Type	Article
Subject	Chemistry Materials Science

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