NDLI: Visualization of coral host–pathogen interactions using a stable GFP-labeled Vibrio coralliilyticus strain

Content Provider	Springer Nature Link
Author	Pollock, F. Joseph Krediet, Cory J. Garren, Melissa Stocker, Roman Winn, Karina Wilson, Bryan Huete Stauffer, Carla Willis, Bette L. Bourne, David G.
Copyright Year	2015
Abstract	The bacterium Vibrio coralliilyticus has been implicated as the causative agent of coral tissue loss diseases (collectively known as white syndromes) at sites across the Indo-Pacific and represents an emerging model pathogen for understanding the mechanisms linking bacterial infection and coral disease. In this study, we used a mini-Tn7 transposon delivery system to chromosomally label a strain of V. coralliilyticus isolated from a white syndrome disease lesion with a green fluorescent protein gene (GFP). We then tested the utility of this modified strain as a research tool for studies of coral host–pathogen interactions. A suite of biochemical assays and experimental infection trials in a range of model organisms confirmed that insertion of the GFP gene did not interfere with the labeled strain’s virulence. Using epifluorescence video microscopy, the GFP-labeled strain could be reliably distinguished from non-labeled bacteria present in the coral holobiont, and the pathogen’s interactions with the coral host could be visualized in real time. This study demonstrates that chromosomal GFP labeling is a useful technique for visualization and tracking of coral pathogens and provides a novel tool to investigate the role of V. coralliilyticus in coral disease pathogenesis.
Starting Page	655
Ending Page	662
Page Count	8
File Format	PDF
ISSN	07224028
Journal	Coral Reefs
Volume Number	34
Issue Number	2
e-ISSN	14320975
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-02-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vibrio coralliilyticus Green fluorescent protein Coral disease Host–pathogen interactions Pathogens Bacteria Freshwater & Marine Ecology Oceanography
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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