NDLI: Characterisation of coral explants: a model organism for cnidarian

Content Provider	Springer Nature Link
Author	Gardner, S. G. Nielsen, D. A. Petrou, K. Larkum, A. W. D. Ralph, P. J.
Copyright Year	2014
Abstract	Coral cell cultures made from reef-building scleractinian corals have the potential to aid in the pursuit of understanding of the cnidarian–dinoflagellate symbiosis. Various methods have previously been described for the production of cell cultures in vitro with a range of success and longevity. In this study, viable tissue spheroids containing host tissue and symbionts (coral explants) were grown from the tissues of Fungia granulosa. The cultured explants remained viable for over 2 months and showed morphological similarities in tissue structure and internal microenvironment to reef-building scleractinian corals. The photophysiology of the explants (1 week old) closely matched that of the parent coral F. granulosa. This study provides the first empirical basis for supporting the use of coral explants as laboratory models for studying coral symbioses. In particular, it highlights how these small, self-sustaining, skeleton-free models can be useful for a number of molecular, genetic and physiological analyses necessary for investigating host–symbiont interactions at the microscale.
Starting Page	133
Ending Page	142
Page Count	10
File Format	PDF
ISSN	07224028
Journal	Coral Reefs
Volume Number	34
Issue Number	1
e-ISSN	14320975
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-11-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Explant culture Cnidarian–dinoflagellate symbiosis Symbiodinium Photophysiology Respiration Freshwater & Marine Ecology Oceanography
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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