NDLI: External carbonic anhydrase in three Caribbean corals: quantification of activity and role in CO2 uptake

Content Provider	Springer Nature Link
Author	Tansik, Anna L. Fitt, William K. Hopkinson, Brian M.
Copyright Year	2015
Abstract	Scleractinian corals have complicated inorganic carbon (C i) transport pathways to support both photosynthesis, by their symbiotic dinoflagellates, and calcification. The first step in C i acquisition, uptake into the coral, is critical as the diffusive boundary layer limits the supply of CO2 to the surface and HCO3 − uptake is energy intensive. An external carbonic anhydrase (eCA) on the oral surface of corals is thought to facilitate CO2 uptake by converting HCO3 − into CO2, helping to overcome the limitation imposed by the boundary layer. However, this enzyme has not yet been identified or detected in corals, nor has its activity been quantified. We have developed a method to quantify eCA activity using a reaction–diffusion model to analyze data on 18O removal from labeled C i. Applying this technique to three species of Caribbean corals (Orbicella faveolata, Porites astreoides, and Siderastrea radians) showed that all species have eCA and that the potential rates of CO2 generation by eCA greatly exceed photosynthetic rates. This demonstrates that eCA activity is sufficient to support its hypothesized role in CO2 supply. Inhibition of eCA severely reduces net photosynthesis in all species (on average by 46 ± 27 %), implying that CO2 generated by eCA is a major carbon source for photosynthesis. Because of the high permeability of membranes to CO2, CO2 uptake is likely driven by a concentration gradient across the cytoplasmic membrane. The ubiquity of eCA in corals from diverse genera and environments suggests that it is fundamental for photosynthetic CO2 supply.
Starting Page	703
Ending Page	713
Page Count	11
File Format	PDF
ISSN	07224028
Journal	Coral Reefs
Volume Number	34
Issue Number	3
e-ISSN	14320975
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-04-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Coral Carbonic anhydrase Photosynthesis CO2 Freshwater & Marine Ecology Oceanography
Content Type	Text
Resource Type	Report
Subject	Aquatic Science

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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