NDLI: Preservation of corals in salt-saturated DMSO buffer is superior to ethanol for PCR experiments

Content Provider	Springer Nature Link
Author	Gaither, M. R. Szabó, Z. Crepeau, M. W. Bird, C. E. Toonen, R. J.
Copyright Year	2010
Abstract	Specimen collection is time consuming and expensive, yet few laboratories test preservation methods before setting out on field expeditions. The most common preservation buffer used for coral specimens is >70% EtOH. However, alternatives exist that are less flammable, easier to ship, and are widely used in other taxa. Here, we compare the effects of salt-saturated DMSO (SSD) and EtOH preservation buffers on post-extraction DNA quantity and quality. We found that soft tissue integrity was better maintained and higher quantities of DNA were extracted from EtOH-preserved specimens; however, by all other measures, SSD was a superior preservative to EtOH. Extractions of SSD-preserved specimens resulted in higher molecular weight DNA, higher PCR success, and more efficient amplification than specimens preserved in EtOH. Our results show that SSD is generally a superior preservative to EtOH for specimens destined for PCR studies, but species-specific differences indicate that preservation comparisons should be undertaken before collection and storage of samples.
Starting Page	329
Ending Page	333
Page Count	5
File Format	PDF
ISSN	07224028
Journal	Coral Reefs
Volume Number	30
Issue Number	2
e-ISSN	14320975
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-10-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Preservation methods Real-time quantitative PCR qPCR Scleractinian coral Storage solutions Oceanography Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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