NDLI: Analysis of candidate imprinted genes linked to Dlk1-Gtl2 using a congenic mouse line

Content Provider	Springer Nature Link
Author	Yevtodiyenko, Aleksey Carr, Michael S. Patel, Nafisa Schmidt, Jennifer V.
Copyright Year	2002
Abstract	The study of genomic imprinting requires the use of DNA sequence polymorphisms between interfertile mouse species or strains. Most commonly, crosses between Mus musculus domesticus and Mus musculus castaneus or Mus spretus animals are used. Difficulties arise in the maintenance of these wild-derived mice in conventional animal facilities, however, and can be overcome by the use of a congenic strain for the region under study. We describe here the generation of a new mouse line, congenic for a region on distal Chromosome (Chr) 12 that encompasses the Dlk1–Gtl2 imprinted domain. We have taken a first step towards demonstrating the utility of these animals by assaying known genes located within the congenic interval for imprinted expression. We show that the two genes located immediately proximal to Dlk1, the Yy1 and Wars genes, are expressed in a biallelic manner. In addition, we have analyzed the Dio3 gene, located distal to Gtl2. This gene displays preferential expression of the paternal allele, with approximately 75% of the total message level originating from the paternal allele and 25% originating from the maternal allele. These data delineate the position of the Wars gene as the proximal boundary of the Dlk1–Gtl2 imprinted domain, and identify Dio3 as another potentially imprinted gene within this domain.
Starting Page	633
Ending Page	638
Page Count	6
File Format	PDF
ISSN	09388990
Journal	Mammalian Genome
Volume Number	13
Issue Number	11
e-ISSN	14321777
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-11-01
Publisher Place	New York, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics

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