NDLI: Gene-environment interactions in a mutant mouse kindred with native airway constrictor hyperresponsiveness

Content Provider	Springer Nature Link
Author	Pinto, Lawrence H. Eaton, Emily Chen, Bohao Fleisher, Jonah Shuster, Dmitry McCauley, Joel Kedainis, Dalius Siepka, Sandra M. Shimomura, Kazuhiro Song, Eun Joo Husain, Aliya Lakser, Oren J. Mitchell, Richard W. Dowell, Maria L. Brown, Melanie Camoretti Mercado, Blanca Naclerio, Robert Sperling, Anne I. Levin, Stephen I. Turek, Fred W. Solway, Julian
Copyright Year	2007
Abstract	We mutagenized male BTBR mice with N-ethyl-N-nitrosourea and screened 1315 of their G3 offspring for airway hyperresponsiveness. A phenovariant G3 mouse with exaggerated methacholine bronchoconstrictor response was identified and his progeny bred in a nonspecific-pathogen-free (SPF) facility where sentinels tested positive for minute virus of mice and mouse parvovirus and where softwood bedding was used. The mutant phenotype was inherited through G11 as a single autosomal semidominant mutation with marked gender restriction, with males exhibiting almost full penetrance and very few females phenotypically abnormal. Between G11 and G12, facility infection eradication was undertaken and bedding was changed to hardwood. We could no longer detect airway hyperresponsiveness in more than 37 G12 offspring of 26 hyperresponsive G11 males. Also, we could not identify the mutant phenotype among offspring of hyperresponsive G8–G10 sires rederived into an SPF facility despite 21 attempts. These two observations suggest that both genetic and environmental factors were needed for phenotype expression. We suspect that rederivation into an SPF facility or altered exposure to pathogens or other unidentified substances modified environmental interactions with the mutant allele, and so resulted in disappearance of the hyperresponsive phenotype. Our experience suggests that future searches for genes that confer susceptibility for airway hyperresponsiveness might not be able to identify some genes that confer susceptibility if the searches are performed in SPF facilities. Experimenters are advised to arrange for multigeneration constancy of mouse care in order to clone mutant genes. Indeed, we were not able to map the mutation before losing the phenotype.
Starting Page	2
Ending Page	14
Page Count	13
File Format	PDF
ISSN	09388990
Journal	Mammalian Genome
Volume Number	19
Issue Number	1
e-ISSN	14321777
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Zoology Anatomy Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics

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