NDLI: Bovine β-defensins: Identification and characterization of novel bovine β-defensin genes and their expression in mammarygland tissue

Content Provider	Springer Nature Link
Author	Roosen, Susanne Exner, Kay Paul, Sven Schröder, Jens Michael Kalm, Ernst Looft, Christian
Copyright Year	2004
Abstract	β-Defensin genes code for multifunctional peptides with a broad-range antimicrobial activity. In this project we hypothesized that β-defensin genes may be candidate genes for resistance to mastitis. In this article we describe the identification and genomic characterization of eight bovine β-defensin genes, including six novel defensin genes and two pseudogenes. Expression in the bovine mammary gland of one of the novel genes, DEFB401, has been demonstrated, as well as the expression of LAP, TAP, DEFB1, BNBD3, BNBD9, and BNBD12. For genomic characterization, 20 BACs from two different bovine BAC libraries (RZPD numbers 750 and 754) were isolated by PCR screening with β-defensin consensus primers derived from published sequences. PCR products from BACs generated with consensus primers have been subcloned and sequenced, revealing a total of 16 genes and two pseudogenes. Six novel β-defensin genes share the typical exon–intron structure and are highly homologous to published bovine β-defensin genes. They are named DEFB401–DEFB405 and LAP-like, and two novel pseudogenes are named EBD-P and EBD-P2. Analysis of mammary gland tissue-derived cDNA from nine cows with different clinical findings demonstrated the expression of several β-defensin genes mentioned above. First results indicate that the lactational status of the cow presumably has no influence on gene expression. Competent knowledge of antimicrobial activity of β-defensins from literature, the abundance of β-defensin mRNA in the bovine mammary gland, and the inducibility of some genes give first evidence that β-defensins may play a role in local host defense during udder infections.
Starting Page	834
Ending Page	842
Page Count	9
File Format	PDF
ISSN	09388990
Journal	Mammalian Genome
Volume Number	15
Issue Number	10
e-ISSN	14321777
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anatomy Cell Biology Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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