NDLI: Genetic alterations at the Bpag1 locus in dt mice and their impact on transcript expression

Content Provider	Springer Nature Link
Author	Pool, Madeline Boudreau Larivière, Céline Bernier, Gilbert Young, Kevin G. Kothary, Rashmi
Copyright Year	2005
Abstract	The dystonin/Bpag1 gene encodes several tissue-specific alternatively spliced transcripts that encode cytoskeletal binding proteins. These various isoforms are necessary for maintaining the structural integrity of epithelial, neural, and muscle tissues. Mutations in the dystonin/Bpag1 gene cause dystonia musculorum (dt), a hereditary neuropathy of the mouse characterized by the progressive degeneration of sensory neurons. Several dt mutant alleles exist, most of which have arisen through spontaneous mutations. In this article we demonstrate that the dt locus encodes 107 exons spanning 400 kb. The high frequency of occurrence of spontaneous dt mutants may therefore be a result of the large size of the gene. Analysis of genomic DNA from several dt spontaneous mutant alleles, dt24J, dt27J, dtAlb, and dtFrk, shows a deletion of the central portion of the gene in dtAlb but no large rearrangements or deletions in the other alleles. These other alleles likely have small deletions or rearrangements, or point mutations. To determine the impact of the known and unknown mutations on transcript levels, RT-PCR was performed to detect various coding regions of the dystonin/Bpag1 transcripts in brain and muscle from multiple dt alleles: dtTg4, dtAlb, dt24J, dt27J, and dtFrk. With the exception of dtFrk, reduced transcript levels were observed for all alleles tested. Such alterations likely result in reduced or absent dystonin/Bpag1 protein levels. Thus, distinct genetic defects lead to a common outcome of reduced transcript expression causing the same phenotype in multiple dt alleles.
Starting Page	909
Ending Page	917
Page Count	9
File Format	PDF
ISSN	09388990
Journal	Mammalian Genome
Volume Number	16
Issue Number	12
e-ISSN	14321777
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-12-08
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anatomy Cell Biology Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics

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