NDLI: Purification and characterization of a psychrophilic glutathione reductase from Antarctic ice microalgae Chlamydomonas sp. Strain ICE-L

Content Provider	Springer Nature Link
Author	Ding, Yu Miao, Jin Lai Wang, Quan Fu Zheng, Zhou Li, Guang You Jian, Ji Chang Wu, Zao He
Copyright Year	2007
Abstract	A psychrophilic glutathione reductase from Antarctic ice microalgae Chlamydomonas sp. Strain ICE-L was purified by ammonium sulfate fractionation and three steps of chromatography. The yield was up to 25.1% of total glutathione reductase in the crude enzyme extract. The glutathione reductase activity was characterized by the spectrophotometric method under different conditions. Purified glutathione reductase was separated by SDS-PAGE, which furnished a homogeneous band. The native molecular mass of the enzyme was 115 kDa. Apparent Km values for NADPH and NADH (both at 0.5 mmol L−1 oxidized glutathione) were 22.3 and 83.8 μmol L−1, respectively. It was optimally active at pH 7.5, and it was stable from pH 5 to 9. Its optimum temperature was 25°C, with activity at 0°C 23.5% of the maximum. Its optimum ion strength and optimum Mg2+ were 50–90 and 7.5 mmol L−1, respectively. Ca2+, Mg2+, and cysteine substantially increased the activity of the enzyme but chelating agents, heavy metals (Cd2+, Pb2+, Cu2+, Zn2+, etc.), NADPH, and ADP had significant inhibitory effects. This glutathione reductase can be used to study the adaptation and mechanism of catalysis of psychrophilic enzymes, and it has a high potential as an environmental biochemical indicator under extreme conditions.
Starting Page	23
Ending Page	30
Page Count	8
File Format	PDF
ISSN	07224060
Journal	Polar Biology
Volume Number	31
Issue Number	1
e-ISSN	14322056
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-08-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chlamydomonas sp. strain ICE-L Glutathione reductase Purification Function characterization Inhibitory effect Zoology Plant Sciences Microbiology Oceanography Ecology
Content Type	Text
Resource Type	Article
Subject	Agricultural and Biological Sciences

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