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| Content Provider | Springer Nature Link |
|---|---|
| Author | Schiaffi, M. Romina Unrein, Fernando Gasol, Josep M. Farias, María E. Estevez, Cristina Balagué, Vanessa Izaguirre, Irina |
| Copyright Year | 2009 |
| Abstract | The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing during two consecutive austral summers (2003–2004). Analyses of the gels identified a total of 230 bands spread across 57 different positions. Among those bands, 14 were shared between lakes from Hope Bay and Potter Peninsula, 17 were observed only in particular lakes, and 17 were registered both years in the same lake. We successfully reamplified and sequenced 43 bands located in 36 different positions belonging to Bacteroidetes, Actinobacteria, Betaproteobacteria and Cyanobacteria. The closest matches for 63% of the sequenced bands were from Antarctic or from other cold environment clones and sequences already in the databases, suggesting the widespread dominance of microbial communities adapted to cold habitats. The results of the multivariate analyses (Cluster Analysis and CCA) indicated that the nutrient status of the lake influences the bacterioplankton assemblages. |
| Starting Page | 923 |
| Ending Page | 936 |
| Page Count | 14 |
| File Format | |
| ISSN | 07224060 |
| Journal | Polar Biology |
| Volume Number | 32 |
| Issue Number | 6 |
| e-ISSN | 14322056 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2009-03-13 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Maritime Antarctic lakes Bacterioplankton 16S rRNA gene Denaturing gradient gel electrophoresis Hope Bay (Antarctic Peninsula) Potter Peninsula (King George Island) Zoology Ecology Plant Sciences Microbiology Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Agricultural and Biological Sciences |
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