NDLI: Mass propagation of ornamental gentian in liquid medium

Content Provider	Springer Nature Link
Author	Hosokawa, K. Oikawa, Y. Yamamura, S.
Copyright Year	1998
Abstract	An efficient system for clonal mass propagation in liquid culture was established for the propagation of ornamental gentian. In a test of the requirements for three cytokinins [6-benzylaminopurine, N-(2-chloro-4-pyridyl)-N′-phenylurea and N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ)] in combination with 1-naphthaleneacetic acid (NAA), we found that effective propagation of shoots occurred with 0.01 mg l–1 TDZ in a 300 ml conical flask that contained 100 ml of medium. The propagation of shoots was also affected by the concentrations of macronutrients (KNO3, NH4NO3 and CaCl2) and sucrose in Murashige and Skoog's (MS) medium, and it was influenced to some extent by the speed of agitation on an orbital shaker. The most efficient propagation of shoots was achieved in full-strength MS medium supplemented with 0.01 mg l–1 TDZ and 20 g l–1 sucrose with agitation at 150 rpm. The propagation of shoots was maximal after 6 weeks of culture (140 shoots from five nodal segments in one flask). Large-scale propagation in a 5-l fermenter was attempted using 3 l of MS medium that contained 0.01 mg l–1 TDZ and 20 g l–1 sucrose. More than 2,000 shoots were obtained in the fermenter in 5 weeks following the initial cultivation of five nodal segments for 6 weeks in one 300-ml flask. The shoots that had propagated in the fermenter were transferred directly to soil without prior rooting in vitro and were easily acclimatized within 1 month.
Starting Page	747
Ending Page	751
Page Count	5
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	17
Issue Number	10
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-07-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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