NDLI: Enhanced production and germination of somatic embryos by temporary starvation in tissue cultures of Daucus carota

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Enhanced production and germination of somatic embryos by temporary starvation in tissue cultures of Daucus carota

Content Provider	Springer Nature Link
Author	Lee, E. K. Cho, D. Y. Soh, W. Y.
Copyright Year	2001
Abstract	Embryogenic callus was induced from cotyledonary explants of Daucus carota L. cultured on solidified MS medium supplemented with 1 mg l–1 2,4-D. Following callus initiation somatic embryos were developed from the callus on MS medium without 2,4-dichlorophenoxyacetic acid. To stimulate the production and germination of somatic embryos we cultured the callus under physically and chemically modified conditions during subculture. When the embryogenic callus was cultured on half-strength MS medium or MS medium without sucrose or cultured under conditions of reduced humidity (69.3%), the production of embryos increased 3.4- to 4.5-fold compared to culture on MS medium containing 3% sucrose (control). Embryogenic callus cultured on MS medium after 5 days of starvation (by being placed in empty 12-well tissue culture plates) showed a 20-fold increase in somatic embryo production and enhanced maturation and germination of embryos. An important point is that the germination of somatic embryos with cup-shaped cotyledons, after a period in culture without medium, was remarkably improved (92%) compared to that of the controls (23%).Thus, we were able to show that stress by starvation without medium led to the enhanced production and increased germination of somatic embryos.
Starting Page	408
Ending Page	415
Page Count	8
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	20
Issue Number	5
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2014-02-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell Biology Plant Sciences Biotechnology Plant Biochemistry
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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