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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhao, Zhi gang Hu, Ting ting Ge, Xian Hong Du, Xue zhu Ding, Li Li, Zai yun |
| Copyright Year | 2008 |
| Abstract | Alien chromosome addition lines have been widely used for identifying gene linkage groups, assigning species-specific characters to a particular chromosome and comparing gene synteny between related species. In plant breeding, their utilization lies in introgressing characters of agronomic value. The present investigation reports the production of intergeneric somatic hybrids Brassica napus (2n = 38) + Orychophragmus violaceus (2n = 24) through asymmetric fusions of mesophyll protoplasts and subsequent development of B. napus-O. violaceous chromosome addition lines. Somatic hybrids showed variations in morphology and fertility and were mixoploids (2n = 51–67) with a range of 19–28 O. violaceus chromosomes identified by genomic in situ hybridization (GISH). After pollinated with B. napus parent and following embryo rescue, 20 BC1 plants were obtained from one hybrid. These exhibited typical serrated leaves of O. violaceus or B. napus-type leaves. All BC1 plants were partially male fertile but female sterile because of abnormal ovules. These were mixoploids (2n = 41–54) with 9–16 chromosomes from O. violaceus. BC2 plants showed segregations for female fertility, leaf shape and still some chromosome variation (2n = 39–43) with 2–5 O. violaceus chromosomes, but mainly containing the whole complement from B. napus. Among the selfed progenies of BC2 plants, monosomic addition lines (2n = 39, AACC + 1O) with or without the serrated leaves of O. violaceus or female sterility were established. The complete set of additions is expected from this investigation. In addition, O. violaceus plants at diploid and tetraploid levels with some variations in morphology and chromosome numbers were regenerated from the pretreated protoplasts by iodoacetate and UV-irradiation. |
| Starting Page | 1611 |
| Ending Page | 1621 |
| Page Count | 11 |
| File Format | |
| ISSN | 07217714 |
| Journal | Plant Cell Reports |
| Volume Number | 27 |
| Issue Number | 10 |
| e-ISSN | 1432203X |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2008-07-15 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Brassica napus Orychophragmus violaceus Somatic hybrids Genomic in situ hybridization (GISH) Monosomic addition Plant Biochemistry Biotechnology Plant Sciences Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Medicine Agronomy and Crop Science |
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