NDLI: High efficiency inducible gene expression system based on activation of a chimeric transcription factor in transgenic pine

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High efficiency inducible gene expression system based on activation of a chimeric transcription factor in transgenic pine

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High efficiency inducible gene expression system based on activation of a chimeric transcription factor in transgenic pine

Content Provider	Springer Nature Link
Author	Tang, Wei Newton, Ronald J. Charles, Thomas M.
Copyright Year	2005
Abstract	Inducible gene expression systems are needed in functional genomics of tree species. A glucocorticoid-inducible gene expression system was established in a gymnosperm species Virginia pine (Pinus virginiana Mill.) through Agrobacterium tumefaciens-mediated genetic transformation. The results demonstrate that expression of the m-gfp5-ER reporter gene was tightly controlled and 0.1 μM of the glucocorticoid hormone triamcinolone was able to induce m-gfp5-ER expression in transgenic cells. Differential expression of gfp in transgenic cells induced by different concentrations of triamcinolone was observed and confirmed by Northern Blot analysis and by quantitative green fluorescence analyses with Laser Scanning Microscopy. In transgenic plantlets, triamcinolone was taken up efficiently by roots. Triamcinolone was able to induce m-gfp5-ER activity throughout the whole plant. The phenotype of transgenic plantlets was not affected 6 weeks after treatment with 0.1–10 μM triamcinolone. However, 6-week inductions with 100 μM triamcinolone caused growth retardation and developmental defects, as well as inhibition of root formation and elongation. With careful selection of transgenic lines, the inducible gene expression presented in this study could be a very valuable alternative for functional identification of novel genes in plants, especially in pine.
Starting Page	619
Ending Page	628
Page Count	10
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	24
Issue Number	10
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-08-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chimeric transcription factor Inducible gene expression Triamcinolone Green fluorescence protein Pinus virginiana Mill Plant Biochemistry Biotechnology Plant Sciences Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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