NDLI: Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Helianthus annuus L.

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Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Helianthus annuus L.

Content Provider	Springer Nature Link
Author	Dhaka, N. Kothari, S.
Copyright Year	2002
Abstract	We have developed a highly efficient three-stage protocol for plant regeneration in sunflower (Helianthus annuus L.) from embryonal cotyledons. This protocol uses phenylacetic acid (PAA) for both shoot-bud induction and the elongation of smaller buds. The medium used for inducing bud formation from the cotyledons was modified MS medium supplemented with 3 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l PAA. Buds were elongated on MS medium supplemented either with only 0.2 mg/l gibberellic acid (GA3) or with 0.2 mg/l GA3 + 0.1 mg/l PAA + 0.3 mg/l BAP. The elongated shoots were then transferred onto rooting medium containing 1 mg/l PAA. The complete plantlets with well-developed roots were transferred to field conditions where they survived and set normal seeds. The induction of shoot buds from embryonal cotyledons was also observed on modified MS medium supplemented with 0.5–5 mg/l BAP in combination with 0.5–5 mg/l α-naphthaleneacetic acid (NAA). In this case, the formation of callus took place along with shoot-bud formation, which hindered further development of the latter. The presence of PAA with BAP in the primary bud induction medium promoted normal development and elongation of shoot buds.
Starting Page	29
Ending Page	34
Page Count	6
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	21
Issue Number	1
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-05-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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