NDLI: Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.)

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Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.)

Content Provider	Springer Nature Link
Author	Ye, Rongjian Zhou, Fei Lin, Yongjun
Copyright Year	2012
Abstract	In plant genetic engineering, using tissue-specific promoters to control the expression of target gene is an effective way to avoid potential negative effects of using constitutive promoter, such as metabolic burden and so on. However, until now, there are few tissue-specific promoters with strong and reliable expression that could be used in crop biotechnology application. In this study, based on microarray and RT-PCR data, we identified a rice green tissue-specific expression gene DX1 (LOC_Os12g33120). The expression pattern of DX1 gene promoter was examined by using the β-glucuronidase (GUS) reporter gene and analyzed in transgenic rice plants in different tissues. Histochemical assays and quantitative analyses of GUS activity confirmed that P DX1 :GUS was highly expressed in green tissues. To identify the regulatory elements controlling the expression of the DX1 gene, a series of 5′ and 3′ deletions of DX1 promoter were fused to GUS gene and stably introduced into rice plants. In addition, gel mobility shift assays and site-directed mutagenesis studies were used, allowing for the identification of two novel tissue-specific cis-acting elements (GSE1 and GSE2) within P DX1 . GSE1 acted as a positive regulator in all green tissues (leaf, sheath, stem and panicle). Compared with GSE1, GSE2 acted as a positive regulator only in sheath and stem tissue, and had a weaker effect on gene expression. In addition, P DX1 :GUS was not expressed in anther and seed, this characteristic reduced the potential ecological risk and potential food safety issues. Taken together, our results strongly suggest that the identified promoter, P DX1 , and its cis regulatory elements, GSE1 and GSE2, are potentially useful in the field of rice transgenic breeding.We have isolated and characterized the rice green tissue-specific promoter P DX1 , and identified two novel positive cis-acting elements in P DX1 .
Starting Page	1159
Ending Page	1172
Page Count	14
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	31
Issue Number	7
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-03-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Promoter cis-element Green tissue-specific GUS gene Rice Plant Biochemistry Cell Biology Biotechnology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.).

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