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Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators

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Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators

Content Provider	Springer Nature Link
Author	Dhar, Uppeandra Joshi, Mitali
Copyright Year	2005
Abstract	A callus induction and in vitro plantlet regeneration system for the endangered state flower of Uttaranchal (Saussurea obvallata) was optimized by studying the influence of explant type (root, hypocotyl, cotyledon and leaf), age and different concentrations of plant growth regulators. Explants from 10 to 15-day-old seedlings showed maximum callus induction. Callus formation and shoot differentiation was initiated on Murashige-Skoog (MS) medium containing 6-benzyladenine (BA) and α-naphthalene acetic acid (NAA) in all explant types. The best results were obtained using leaf explants: 100% callusing was achieved in MS medium supplemented with 2.5 μM BA and 1.0 μM NAA, and 100% differentiation along with a multiplication rate of 12 shoots per explant with a combination of 5.0 μM BA and 1.0 μM NAA. However, the results reflected the existence of high inter-explant variability in response to growth regulators. In vitro rooting of shoots was achieved at an efficiency of 100% in one-half strength MS medium supplemented with 2.5 μM indole-3-butyric acid. Application of this protocol has potential for mass multiplication of the target species in a limited time period.
Starting Page	195
Ending Page	200
Page Count	6
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	24
Issue Number	4
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-03-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Asteraceae Callus Leaf explant Saussurea obvallata
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators

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Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators