NDLI: Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate

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Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate

Content Provider	Springer Nature Link
Author	Chhapekar, Sushil Raghavendrarao, Sanagala Pavan, Gadamchetty Ramakrishna, Chopperla Singh, Vivek Kumar Phanindra, Mullapudi Lakshmi Venkata Dhandapani, Gurusamy Sreevathsa, Rohini Ananda Kumar, Polumetla
Copyright Year	2014
Abstract	Highly tolerant herbicide-resistant transgenic rice was developed by expressing codon-modified synthetic CP4 - EPSPS . The transformants could tolerate up to 1 % commercial glyphosate and has the potential to be used for DSR (direct-seeded rice). Weed infestation is one of the major biotic stress factors that is responsible for yield loss in direct-seeded rice (DSR). Herbicide-resistant rice has potential to improve the efficiency of weed management under DSR. Hence, the popular indica rice cultivar IR64, was genetically modified using Agrobacterium-mediated transformation with a codon-optimized CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene, with N-terminal chloroplast targeting peptide from Petunia hybrida. Integration of the transgenes in the selected rice plants was confirmed by Southern hybridization and expression by Northern and herbicide tolerance assays. Transgenic plants showed EPSPS enzyme activity even at high concentrations of glyphosate, compared to untransformed control plants. T 0, T 1 and T 2 lines were tested by herbicide bioassay and it was confirmed that the transgenic rice could tolerate up to 1 % of commercial Roundup, which is five times more in dose used to kill weeds under field condition. All together, the transgenic rice plants developed in the present study could be used efficiently to overcome weed menace.
Starting Page	721
Ending Page	731
Page Count	11
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	34
Issue Number	5
e-ISSN	1432203X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-12-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Codon optimization Direct-seeded rice (DSR) 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) Glyphosate resistance Weed management Cell Biology Plant Sciences Biotechnology Plant Biochemistry
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate.

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Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate

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Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate.

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Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate