NDLI: High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos

Please wait, while we are loading the content...

High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos

Microprojectile-mediated transient and integrative transformation of rice embryogenic suspension cells: effects of osmotic cell conditioning and of the physical configuration of plasmid DNA

Dithiothreitol increases b-glucuronidase accumulation in transformed tobacco (Nicotiana tabacum) protoplasts without altering their viability or the synthesis and export of cellular proteins

Glucosylation of ethanol in Ilex paraguariensis cell suspension cultures

An improved transformation protocol for studying gene expression in hairy roots of sugar beet (Beta vulgaris L.)

Detection of somaclonal variation in cultured rice cells using digoxigenin-based random amplified polymorphic DNA

Plant Cell Reports : Volume 18, Issue 5, January 1999

Plant Cell Reports : Volume 18, Issue 3-4, December 1998

Plant Cell Reports : Volume 18, Issue 1-2, November 1998

Plant Cell Reports : Volume 17

Plant Cell Reports : Volume 16

High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos

Content Provider	Springer Nature Link
Author	Choi, Y. E. Yang, D. C. Yoon, E. S. Choi, K. T.
Copyright Year	1999
Abstract	Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly, most in a multiple state, fused together and to the parent cotyledon explants. When the cotyledon explants of ginseng were pretreated with 1.0 m sucrose for 24–72 h, all the somatic embryos developed individually from all surfaces of the cotyledons and the number of somatic embryos per explant was enhanced fourfold. Histological observation revealed that all the single somatic embryos from preplasmolysed cotyledons originated from epidermal single cells, whereas all the multiple embryos from cotyledons without pretreatment originated from epidermal and subepidermal cell masses. When the somatic embryos matured to the cotyledonary stage, further growth ceased and they remained white, probably indicating dormancy. Gibberellic acid (GA3) (over 1.0 mg/l) or chilling treatment (–2°C for over 8 weeks) were prerequisites for the germination of somatic embryos. Ultrastructural observation revealed that the cotyledon cells of somatic embryos without chilling or GA3 treatment contained numerous lipid reserves, dense cytoplasm, proplastids and non-activated mitochondria, whereas the cotyledon cells of somatic embryos after chilling or GA3 treatment were highly vacuolated and contained well-developed chloroplasts and active-state mitochondria enclosing numerous cristae, indicating that in-vitro-developed somatic embryos of P. ginseng may be dormant after maturing in a manner similar to zygotic embryos.
Starting Page	493
Ending Page	499
Page Count	7
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	18
Issue Number	6
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-02-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants

Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis.

High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng

Regenerative ability of somatic single and multiple embryos from cotyledons of Korean ginseng on hormone-free medium

High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis

Plant regeneration from protoplasts of immature Vigna sinensis cotyledons via somatic embryogenesis.

Optimization of direct somatic embryogenesis from mature zygotic embryos ofPanax ginseng C. A. Meyer

Distribution and changes of reserve materials in cotyledon cells of Panax ginseng related to direct somatic embryogenesis and germination

High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos

Similar Documents

Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants

Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis.

High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng

Regenerative ability of somatic single and multiple embryos from cotyledons of Korean ginseng on hormone-free medium

High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis

Plant regeneration from protoplasts of immature Vigna sinensis cotyledons via somatic embryogenesis.

Optimization of direct somatic embryogenesis from mature zygotic embryos ofPanax ginseng C. A. Meyer

Distribution and changes of reserve materials in cotyledon cells of Panax ginseng related to direct somatic embryogenesis and germination

High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos