NDLI: Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot

Please wait, while we are loading the content...

Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot

Characterization of five polyamine oxidase isoforms in Arabidopsis thaliana

Cucumber mosaic virus 2b protein inhibits RNA silencing pathways in green alga Chlamydomonas reinhardtii

Overexpression of a homopeptide repeat-containing bHLH protein gene (OrbHLH001) from Dongxiang Wild Rice confers freezing and salt tolerance in transgenic Arabidopsis

Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein

Genetic transformation of sweet sorghum

Shaker-like potassium channels in Populus, regulated by the CBL–CIPK signal transduction pathway, increase tolerance to low-K+ stress

Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA

Recovery of citrus triploid hybrids by embryo rescue and flow cytometry from 2x × 2x sexual hybridisation and its application to extensive breeding programs

Constitutive expression of a fungal glucose oxidase gene in transgenic tobacco confers chilling tolerance through the activation of antioxidative defence system

GA3 stimulates the formation and germination of somatic embryos and the expression of a KNOTTED-like homeobox gene of Cocos nucifera (L.)

Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants

Plant Cell Reports : Volume 29, Issue 8, August 2010

Plant Cell Reports : Volume 29, Issue 7, July 2010

Plant Cell Reports : Volume 29, Issue 6, June 2010

Plant Cell Reports : Volume 29, Issue 5, May 2010

Plant Cell Reports : Volume 29, Issue 4, April 2010

Plant Cell Reports : Volume 29, Issue 3, March 2010

Plant Cell Reports : Volume 29, Issue 2, February 2010

Plant Cell Reports : Volume 29, Issue 1, January 2010

Plant Cell Reports : Volume 28

Plant Cell Reports : Volume 27

Plant Cell Reports : Volume 26

Plant Cell Reports : Volume 25

Plant Cell Reports : Volume 24

Plant Cell Reports : Volume 23

Plant Cell Reports : Volume 22

Plant Cell Reports : Volume 21

Plant Cell Reports : Volume 20

Plant Cell Reports : Volume 19

Plant Cell Reports : Volume 18

Plant Cell Reports : Volume 17

Plant Cell Reports : Volume 16

Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot

Content Provider Springer Nature Link
Author Ntui, Valentine Otang Thirukkumaran, Gunaratnam Azadi, Pejman Khan, Raham Sher Nakamura, Ikuo Mii, Masahiro
Copyright Year 2010
Abstract Production of “Egusi” melon (Colocynthis citrullus L.) in West Africa is limited by fungal diseases, such as Alternaria leaf spot and Fusarium wilt. In order to engineer “Egusi” resistant to these diseases, cotyledonary explants of two “Egusi” genotypes, ‘Ejagham’ and NHC1-130, were transformed with Agrobacterium tumefaciens strain EHA101 harbouring wasabi defensin gene (isolated from Wasabia japonica L.) in a binary vector pEKH1. After co-cultivation for 3 days, infected explants were transferred to MS medium containing 100 mgl−l kanamycin to select transformed tissues. After 3 weeks of culture, adventitious shoots appeared directly along the edges of the explants. As much as 19 out of 52 (36.5%) and 25 out of 71 (35.2%) of the explants in genotype NHC1-130 and ‘Ejagham’, respectively, formed shoots after 6 weeks of culture. As much as 74% (14 out of 19) of the shoots regenerated in genotype NHC1-130 and 72% (18 out of 25) of those produced in genotype ‘Ejagham’ were transgenic. A DNA fragment corresponding to the wasabi defensin gene or the selection marker nptII was amplified by PCR from the genomic DNA of all regenerated plant clones rooted on hormone-free MS medium under the same selection pressure, suggesting their transgenic nature. Southern blot analysis confirmed successful integration of 1–5 copies of the transgene. RT-PCR, northern and western blot analyses revealed that wasabi defensin gene was expressed in transgenic lines. Transgenic lines showed increased levels of resistance to Alternaria solani, which causes Alternaria leaf spot and Fusarium oxysporum, which causes Fusarium wilt, as compared to that of untransformed plants.
Starting Page 943
Ending Page 954
Page Count 12
File Format PDF
ISSN 07217714
Journal Plant Cell Reports
Volume Number 29
Issue Number 9
e-ISSN 1432203X
Language English
Publisher Springer-Verlag
Publisher Date 2010-06-15
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Colocynthis citrullus Cotyledon Genetic transformation Antifungal resistance Wasabi defensin Plant Biochemistry Biotechnology Plant Sciences Cell Biology
Content Type Text
Resource Type Article
Subject Plant Science Medicine Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Stable integration and expression of wasabi defensin gene in 'Egusi' melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot.

An efficient Agrobacterium tumefaciens-mediated genetic transformation of “Egusi” melon (Colocynthis citrullus L.)

in “Egusi ” melon (Colocynthis citrullus L.) (2013)

Plant regeneration and Agrobacterium-mediated transformation of cotyledon explants of Citrullus colocynthis (L.) Schrad.

Intraspecific hybridization in “Egusi” melon, Colocynthis citrullus L.

RESPONSES OF EGUSI MELON (COLOCYNTHIS CITROLLUS L) TO POULTRY MANURE IN OBUBRA, CROSS RIVER, SOUTH-SOUTH NIGERIA

Effect of drying method on the quality and storability of 'egusi' melon seeds (Colocynthis citrullus L.)

Expression of hybrid vigour in some yield attributes of Egusi melon(Colocynthis citrullus L)

Antifungal Activity of Nettle (Urtica dioica L.), Colocynth (Citrullus colocynthis L. Schrad), Oleander (Nerium oleander L.) and Konar (Ziziphus spina-christi L.) Extracts on Plants Pathogenic Fungi

Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot

Content Provider	Springer Nature Link
Author	Ntui, Valentine Otang Thirukkumaran, Gunaratnam Azadi, Pejman Khan, Raham Sher Nakamura, Ikuo Mii, Masahiro
Copyright Year	2010
Abstract	Production of “Egusi” melon (Colocynthis citrullus L.) in West Africa is limited by fungal diseases, such as Alternaria leaf spot and Fusarium wilt. In order to engineer “Egusi” resistant to these diseases, cotyledonary explants of two “Egusi” genotypes, ‘Ejagham’ and NHC1-130, were transformed with Agrobacterium tumefaciens strain EHA101 harbouring wasabi defensin gene (isolated from Wasabia japonica L.) in a binary vector pEKH1. After co-cultivation for 3 days, infected explants were transferred to MS medium containing 100 mgl−l kanamycin to select transformed tissues. After 3 weeks of culture, adventitious shoots appeared directly along the edges of the explants. As much as 19 out of 52 (36.5%) and 25 out of 71 (35.2%) of the explants in genotype NHC1-130 and ‘Ejagham’, respectively, formed shoots after 6 weeks of culture. As much as 74% (14 out of 19) of the shoots regenerated in genotype NHC1-130 and 72% (18 out of 25) of those produced in genotype ‘Ejagham’ were transgenic. A DNA fragment corresponding to the wasabi defensin gene or the selection marker nptII was amplified by PCR from the genomic DNA of all regenerated plant clones rooted on hormone-free MS medium under the same selection pressure, suggesting their transgenic nature. Southern blot analysis confirmed successful integration of 1–5 copies of the transgene. RT-PCR, northern and western blot analyses revealed that wasabi defensin gene was expressed in transgenic lines. Transgenic lines showed increased levels of resistance to Alternaria solani, which causes Alternaria leaf spot and Fusarium oxysporum, which causes Fusarium wilt, as compared to that of untransformed plants.
Starting Page	943
Ending Page	954
Page Count	12
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	29
Issue Number	9
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-06-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Colocynthis citrullus Cotyledon Genetic transformation Antifungal resistance Wasabi defensin Plant Biochemistry Biotechnology Plant Sciences Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

Similar Documents

Stable integration and expression of wasabi defensin gene in 'Egusi' melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot.

An efficient Agrobacterium tumefaciens-mediated genetic transformation of “Egusi” melon (Colocynthis citrullus L.)

in “Egusi ” melon (Colocynthis citrullus L.) (2013)

Plant regeneration and Agrobacterium-mediated transformation of cotyledon explants of Citrullus colocynthis (L.) Schrad.

Intraspecific hybridization in “Egusi” melon, Colocynthis citrullus L.

RESPONSES OF EGUSI MELON (COLOCYNTHIS CITROLLUS L) TO POULTRY MANURE IN OBUBRA, CROSS RIVER, SOUTH-SOUTH NIGERIA

Effect of drying method on the quality and storability of 'egusi' melon seeds (Colocynthis citrullus L.)

Expression of hybrid vigour in some yield attributes of Egusi melon(Colocynthis citrullus L)

Antifungal Activity of Nettle (Urtica dioica L.), Colocynth (Citrullus colocynthis L. Schrad), Oleander (Nerium oleander L.) and Konar (Ziziphus spina-christi L.) Extracts on Plants Pathogenic Fungi

Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot