NDLI: Agrobacterium rhizogenes-mediated transformation of Taraxacum platycarpum and changes of morphological characters

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Agrobacterium rhizogenes-mediated transformation of Taraxacum platycarpum and changes of morphological characters

Content Provider	Springer Nature Link
Author	Lee, M. H. Yoon, E. S. Jeong, J. H. Choi, Y. E.
Copyright Year	2004
Abstract	Transformed hairy roots were efficiently induced from seedlings of Taraxacum platycarpum by infection with Agrobacterium rhizogenes 15834. Root explants produced transformed roots at a higher frequency (76.5±3.5%) as compared to stem (32.7±4.8%) or cotyledon (16.2±5.7%). Hairy roots exhibited active elongation with high branching of roots on growth regulator-free medium. The competence of plant regeneration from non-transformed adventitious roots and transformed hairy roots was compared. The frequency of adventitious shoot formation from transformed roots was much higher (88.5±9.8%) than that of non-transformed roots (31.7 ±9.5%) on hormone-free medium. Rooting of hairy root-derived adventitious shoots occurred easily on growth regulator-free medium but no rooting was observed on non-transformed shoots. The stable introduction of rol genes into Taraxacum plants was confirmed by PCR and Southern hybridization. Transgenic plantlets showed considerable differences in their morphology when compared to the corresponding wild-type (non-transgenic) plants. Plantlets formed from transformed roots had numerous fibrous roots with abundant lateral branches instead of the thickened taproots in non-transformed plants. The differences observed may reflect the modification of morphological root characters by introduction of rol genes.
Starting Page	822
Ending Page	827
Page Count	6
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	22
Issue Number	11
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-02-25
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Taraxacum platycarpum Genetic transformation Hairy root Plant regeneration Morphological character
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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