NDLI: Immunogenicity of nuclear-encoded LTB:ST fusion protein from Escherichia coli expressed in tobacco plants

Content Provider	Springer Nature Link
Author	Rosales Mendoza, Sergio Soria Guerra, Ruth E. More Fierros, Leticia Govea Alonso, Dania O. Herrera Díaz, Areli Korban, Schuyler S. Alpuche Solís, Ángel G.
Copyright Year	2011
Abstract	Enterotoxigenic Escherichia coli (ETEC) is one of the main causative agents of diarrhea in infants and for travelers. Inclusion of a heat-stable (ST) toxin into vaccine formulations is mandatory as most ETEC strains can produce both heat-labile (LT) and ST enterotoxins. In this study, a genetic fusion gene encoding for an LTB:ST protein has been constructed and transferred into tobacco via Agrobacterium tumefaciens-mediated transformation. Transgenic tobacco plants carrying the LTB:ST gene are then subjected to GM1-ELISA revealing that the LTB:ST has assembled into pentamers and displays antigenic determinants from both LTB and ST. Protein accumulation of up to 0.05% total soluble protein is detected. Subsequently, mucosal and systemic humoral responses are elicited in mice orally dosed with transgenic tobacco leaves. This has suggested that the plant-derived LTB:ST is immunogenic via the oral route. These findings are critical for the development of a plant-based vaccine capable of eliciting broader protection against ETEC and targeting both LTB and ST. Features of this platform in comparison to transplastomic approaches are discussed.
Starting Page	1145
Ending Page	1152
Page Count	8
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	30
Issue Number	6
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oral immunization Chimeric protein Broader protective vaccine Biotechnology Plant Sciences Plant Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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